Current Problems in Pediatric and Adolescent Health Care
Volume 39, Issue 3 , Pages 61-88 , March 2009

Diagnosing Childhood Tuberculosis: Traditional and Innovative Modalities

References 

  1. World Health Organization (WHO). Global tuberculosis control: surveillance, planning, financing (WHO Report 2008). Geneva, Switzerland: World Health Organization; 2008;
  2. Report WHO 2006 (Global tuberculosis control—surveillance, planning, financing). 2006;Geneva, Switzerland
  3. Steinman RM. The dendritic cell system and its role in immunogenicity. Annu Rev Immunol. 1991;9:271–296
  4. Chan J, Xing Y, Magliozzo RS, Bloom BR. Killing of virulent Mycobacterium tuberculosis by reactive nitrogen intermediates produced by activated murine macrophages. J Exp Med. 1992;175(4):1111–1122
  5. Gordon AH, Hart PD, Young MR. Ammonia inhibits phagosome-lysosome fusion in macrophages. Nature. 1980;286(5768):79–80
  6. Myrvik QN, Leake ES, Wright MJ. Disruption of phagosomal membranes of normal alveolar macrophages by the H37Rv strain of Mycobacterium tuberculosis (A correlate of virulence). Am Rev Respir Dis. 1984;129(2):322–328
  7. Murray HW. Interferon-gamma, the activated macrophage, and host defense against microbial challenge. Ann Intern Med. 1988;108(4):595–608
  8. Sanchez FO, Rodriguez JI, Agudelo G, Garcia LF. Immune responsiveness and lymphokine production in patients with tuberculosis and healthy controls. Infect Immun. 1994;62(12):5673–5678
  9. Smith S, Jacobs RF, Wilson CB. Immunobiology of childhood tuberculosis: a window on the ontogeny of cellular immunity. J Pediatr. 1997;131(1 Pt. 1):16–26
  10. Kaufmann SH. How can immunology contribute to the control of tuberculosis?. Nat Rev Immunol. 2001;1(1):20–30
  11. Flynn JL, Chan J, Triebold KJ, Dalton DK, Stewart TA, Bloom BR. An essential role for interferon gamma in resistance to Mycobacterium tuberculosis infection. J Exp Med. 1993;178(6):2249–2254
  12. Holland SM, Dorman SE, Kwon A, Pitha-Rowe IF, Frucht DM, Gerstberger SM, et al. Abnormal regulation of interferon-gamma, interleukin-12, and tumor necrosis factor-alpha in human interferon-gamma receptor 1 deficiency. J Infect Dis. 1998;178(4):1095–1104
  13. Newport MJ, Huxley CM, Huston S, Hawrylowicz CM, Oostra BA, Williamson R, et al. A mutation in the interferon-gamma-receptor gene and susceptibility to mycobacterial infection. N Engl J Med. 1996;335(26):1941–1949
  14. Cooper AM, Dalton DK, Stewart TA, Griffin JP, Russell DG, Orme IM. Disseminated tuberculosis in interferon gamma gene-disrupted mice. J Exp Med. 1993;178(6):2243–2247
  15. Kaufmann SH, Ladel CH. Role of T cell subsets in immunity against intracellular bacteria: experimental infections of knock-out mice with Listeria monocytogenes and Mycobacterium bovis BCG. Immunobiology. 1994;191(4-5):509–519
  16. Lewinsohn DA, Gennaro ML, Scholvinck L, Lewinsohn DM. Tuberculosis immunology in children: diagnostic and therapeutic challenges and opportunities. Int J Tuberc Lung Dis. 2004;8(5):658–674
  17. van Crevel R, Ottenhoff TH, van der Meer JW. Innate immunity to Mycobacterium tuberculosis. Clin Microbiol Rev. 2002;15(2):294–309
  18. Lesley R, Ramakrishnan L. Insights into early mycobacterial pathogenesis from the zebrafish. Curr Opin Microbiol. 2008;11(3):277–283
  19. Gedde-Dahl T. Tuberculous infection in the light of tuberculin matriculation. Am J Hyg. 1952;56(2):139–214
  20. Miller FJW, Taylor MD. Tuberculosis in children. SR Boston (MA): Little Brown; 1963;
  21. Marais BJ, Pai M. Specimen collection methods in the diagnosis of childhood tuberculosis. Indian J Med Microbiol. 2006;24(4):249–251
  22. Marais BJ, Gie RP, Schaaf HS, Hesseling AC, Obihara CC, Starke JJ, et al. The natural history of childhood intra-thoracic tuberculosis: a critical review of literature from the pre-chemotherapy era. Int J Tuberc Lung Dis. 2004;8(4):392–402
  23. Nemir RL. Perspectives in adolescent tuberculosis: three decades of experience. Pediatrics. 1986;78(3):399–405
  24. Comstock GW, Edwards LB, Livesay VT. Tuberculosis morbidity in the U.S. Navy: its distribution and decline. Am Rev Respir Dis. 1974;110(5):572–580
  25. Alcais A, Fieschi C, Abel L, Casanova JL. Tuberculosis in children and adults: two distinct genetic diseases. J Exp Med. 2005;202(12):1617–1621
  26. Lincoln EM. The value of follow-up studies of children with primary tuberculosis. Am Rev Tuberc. 1951;64(5):499–507
  27. Brailey M. Mortality in tuberculin-positive infants. Bull Johns Hopkins Hosp; 1936;
  28. Lagrange PH, Wargnier A. Immunological aspects of pulmonary tuberculosis in children. Pediatr Pulmonol Suppl. 1995;11:20–22
  29. Klein RB, Fischer TJ, Gard SE, Biberstein M, Rich KC, Stiehm ER. Decreased mononuclear and polymorphonuclear chemotaxis in human newborns, infants, and young children. Pediatrics. 1977;60(4):467–472
  30. Raghunathan R, Miller ME, Everett S, Leake RD. Phagocyte chemotaxis in the perinatal period. J Clin Immunol. 1982;2(3):242–245
  31. Hunt DW, Huppertz HI, Jiang HJ, Petty RE. Studies of human cord blood dendritic cells: evidence for functional immaturity. Blood. 1994;84(12):4333–4343
  32. Antons AK, Wang R, Kalams SA, Unutmaz D. Suppression of HIV-specific and allogeneic T cell activation by human regulatory T cells is dependent on the strength of signals. PLoS ONE. 2008;3(8):e2952
  33. Sullivan SE, Staba SL, Gersting JA, Hutson AD, Theriaque D, Christensen RD, et al. Circulating concentrations of chemokines in cord blood, neonates, and adults. Pediatr Res. 2002;51(5):653–657
  34. Taylor S, Bryson YJ. Impaired production of gamma-interferon by newborn cells in vitro is due to a functionally immature macrophage. J Immunol. 1985;134(3):1493–1497
  35. McKenzie SE, Kline J, Douglas SD, Polin RA. Enhancement in vitro of the low interferon-gamma production of leukocytes from human newborn infants. J Leukoc Biol. 1993;53(6):691–696
  36. Hussey G, Chisholm T, Kibel M. Miliary tuberculosis in children: a review of 94 cases. Pediatr Infect Dis J. 1991;10(11):832–836
  37. Frenkel L, Bryson YJ. Ontogeny of phytohemagglutinin-induced gamma interferon by leukocytes of healthy infants and children: evidence for decreased production in infants younger than 2 months of age. J Pediatr. 1987;111(1):97–100
  38. Lighter J, Rigaud M, Eduardo R, Peng CH, Pollack H. Latent tuberculosis diagnosis in children using the Quantiferon-TB Gold In-Tube test. Pediatrics. 2009;123:30–37
  39. Aubert-Pivert EM, Chedevergne FM, Lopez-Ramirez GM, Colle JH, Scheinmann PL, Gicquel BM, et al. Cytokine transcripts in pediatric tuberculosis: a study with bronchoalveolar cells. Tuber Lung Dis. 2000;80(6):249–258
  40. Marais BJ, Hesseling AC, Gie RP, Schaaf HS, Beyers N. The burden of childhood tuberculosis and the accuracy of community-based surveillance data. Int J Tuberc Lung Dis. 2006;10(3):259–263
  41. Khan K, Wang J, Hu W, Bierman A, Li Y, Gardam M. Tuberculosis infection in the United States: national trends over three decades. Am J Respir Crit Care Med. 2008;177(4):455–460
  42. Gounder CR, Driver CR, Scholten JN, Shen H, Munsiff SS. Tuberculin testing and risk of tuberculosis infection among New York City schoolchildren. Pediatrics. 2003;111(4 Pt. 1):e309–e315
  43. Hsu KH. Contact investigation: a practical approach to tuberculosis eradication. Am J Public Health Nations Health. 1963;53:1761–1769
  44. Cruz AT, Starke JR. Clinical manifestations of tuberculosis in children. Paediatr Respir Rev. 2007;8(2):107–117
  45. Guidelines for the investigation of contacts of persons with infectious tuberculosis (Recommendations from the National Tuberculosis Controllers Association and CDC). MMWR Recomm Rep. 2005;54(RR-15):1–47
  46. American Academy of Pediatrics CoID. Red book. 27th edition. Elk Grove Village (IL): American Academy of Pediatrics; 2006;
  47. Powell DA, Hunt WG. Tuberculosis in children: an update. Adv Pediatr. 2006;53:279–322
  48. Merino JM, Carpintero I, Alvarez T, Rodrigo J, Sanchez J, Coello JM. Tuberculous pleural effusion in children. Chest. 1999;115(1):26–30
  49. Jacobs RF, Starke JR. Tuberculosis in children. Med Clin North Am. 1993;77(6):1335–1351
  50. Farinha NJ, Razali KA, Holzel H, Morgan G, Novelli VM. Tuberculosis of the central nervous system in children: a 20-year survey. J Infect. 2000;41(1):61–68
  51. Starke JR. Mycobacterium tuberculosis. In:  Long SS editors. Principles and Practice of Pediatric Infectious Diseases. 3rd ed.. Philadelphia, PA: Churchill Livingstone; 2008;
  52. Cruz AT, Starke JR. Treatment of tuberculosis in children. Exp Rev Anti Infect Ther. 2008;6(6):939–957
  53. Diagnostic Standards and Classification of Tuberculosis in Adults and Children (This official statement of the American Thoracic Society and the Centers for Disease Control and Prevention was adopted by the ATS Board of Directors, July 1999. This statement was endorsed by the Council of the Infectious Disease Society of America, September 1999). Am J Respir Crit Care Med. 2000;161(4 Pt. 1):1376–1395
  54. Nash DR, Douglass JE. Anergy in active pulmonary tuberculosis (A comparison between positive and negative reactors and an evaluation of 5 TU and 250 TU skin test doses). Chest. 1980;77(1):32–37
  55. Steiner P, Rao M, Victoria MS, Jabbar H, Steiner M. Persistently negative tuberculin reactions: their presence among children with culture positive for Mycobacterium tuberculosis (tuberculin-negative tuberculosis). Am J Dis Child. 1980;134(8):747–750
  56. Huebner RE, Schein MF, Bass JB. The tuberculin skin test. Clin Infect Dis. 1993;17(6):968–975
  57. Hsu KH, Carreon AT, Jeu F, Jenkins DE. Today's concept of the tuberculin test. Dis Chest. 1964;46:648–663
  58. Farhat M, Greenaway C, Pai M, Menzies D. False-positive tuberculin skin tests: what is the absolute effect of BCG and non-tuberculous mycobacteria?. Int J Tuberc Lung Dis. 2006;10(11):1192–1204
  59. Menzies R, Vissandjee B, Amyot D. Factors associated with tuberculin reactivity among the foreign-born in Montreal. Am Rev Respir Dis. 1992;146(3):752–756
  60. Marcus JH, Khassis Y. The tuberculin sensitivity in BCG vaccinated infants and children in Israel. Acta Tuberc Pneumol Scand. 1965;46(2):113–122
  61. Wang L, Turner MO, Elwood RK, Schulzer M, FitzGerald JM. A meta-analysis of the effect of Bacille Calmette Guerin vaccination on tuberculin skin test measurements. Thorax. 2002;57(9):804–809
  62. Grzybowski S, Allen EA. The challenge of tuberculosis in decline (A study based on the epidemiology of tuberculosis in Ontario, Canada). Am Rev Respir Dis. 1964;90:707–720
  63. Diel R, Loddenkemper R, Meywald-Walter K, Niemann S, Nienhaus A. Predictive value of a whole blood IFN-gamma assay for the development of active tuberculosis disease after recent infection with Mycobacterium tuberculosis. Am J Respir Crit Care Med. 2008;177(10):1164–1170
  64. Andersen P, Doherty TM, Pai M, Weldingh K. The prognosis of latent tuberculosis: can disease be predicted?. Trends Mol Med. 2007;13(5):175–182
  65. Menzies D, Pai M, Comstock G. Meta-analysis: new tests for the diagnosis of latent tuberculosis infection: areas of uncertainty and recommendations for research. Ann Intern Med. 2007;146(5):340–354
  66. Connell TG, Curtis N, Ranganathan SC, Buttery JP. Performance of a whole blood interferon gamma assay for detecting latent infection with Mycobacterium tuberculosis in children. Thorax. 2006;61(7):616–620
  67. Hill PC, Brookes RH, Adetifa IM, Fox A, Jackson-Sillah D, Lugos MD, et al. Comparison of enzyme-linked immunospot assay and tuberculin skin test in healthy children exposed to Mycobacterium tuberculosis. Pediatrics. 2006;117(5):1542–1548
  68. Nakaoka H, Lawson L, Squire SB, Coulter B, Ravn P, Brock I, et al. Risk for tuberculosis among children. Emerg Infect Dis. 2006;12(9):1383–1388
  69. Dogra S, Narang P, Mendiratta DK, Chaturvedi P, Reingold AL, Colford JM, et al. Comparison of a whole blood interferon-gamma assay with tuberculin skin testing for the detection of tuberculosis infection in hospitalized children in rural India. J Infect. 2007;54(3):267–276
  70. Detjen AK, Keil T, Roll S, Hauer B, Mauch H, Wahn U, et al. Interferon-gamma release assays improve the diagnosis of tuberculosis and nontuberculous mycobacterial disease in children in a country with a low incidence of tuberculosis. Clin Infect Dis. 2007;45(3):322–328
  71. Liebeschuetz S, Bamber S, Ewer K, Deeks J, Pathan AA, Lalvani A. Diagnosis of tuberculosis in South African children with a T-cell-based assay: a prospective cohort study. Lancet. 2004;364(9452):2196–2203
  72. Luetkemeyer AF, Charlebois ED, Flores LL, Bangsberg DR, Deeks SG, Martin JN, et al. Comparison of an interferon-gamma release assay with tuberculin skin testing in HIV-infected individuals. Am J Respir Crit Care Med. 2007;175(7):737–742
  73. Lawn SD, Bangani N, Vogt M, Bekker LG, Badri M, Ntobongwana M, et al. Utility of interferon-gamma ELISPOT assay responses in highly tuberculosis-exposed patients with advanced HIV infection in South Africa. BMC Infect Dis. 2007;7:99
  74. Brock I, Ruhwald M, Lundgren B, Westh H, Mathiesen LR, Ravn P. Latent tuberculosis in HIV positive, diagnosed by the M. tuberculosis specific interferon-gamma test. Respir Res. 2006;7:56
  75. Ferrara G, Losi M, Roversi P, Fabbri LM, Richeldi L. [New tools for a better diagnosis of latent tuberculosis infection]. Recenti Prog Med. 2006;97(3):123–128
  76. Arend SM, Thijsen SF, Leyten EM, Bouwman JJ, Franken WP, Koster BF, et al. Comparison of two interferon-gamma assays and tuberculin skin test for tracing tuberculosis contacts. Am J Respir Crit Care Med. 2007;175(6):618–627
  77. Mori T, Harada N, Higuchi K, Sekiya Y, Uchimura K, Shimao T. Waning of the specific interferon-gamma response after years of tuberculosis infection. Int J Tuberc Lung Dis. 2007;11(9):1021–1025
  78. Hougardy JM, Schepers K, Place S, Drowart A, Lechevin V, Verscheure V, et al. Heparin-binding-hemagglutinin-induced IFN-gamma release as a diagnostic tool for latent tuberculosis. PLoS ONE. 2007;2(10):e926
  79. Lighter J, Rigaud M, Huie M, Peng C, Pollack H. Chemokine IP-10: An adjunct marker for latent tuberculosis infection in children. IJTLD. 2009;(in press)
  80. Ruhwald M, Peterson J, Kofoed K, Nakaoka H, Cuevas LE, Lawson L, et al. Improving T-cell assays for the diagnosis of Latent TB infection: Potential of a diagnostic test based on IP-10. PLoS ONE. 2008;3(8):e2858
  81. Starke J. Use of the new TB test in children should be limited. AAP News. 2006;27:14–15
  82. Starke JR, Taylor-Watts KT. Tuberculosis in the pediatric population of Houston, Texas. Pediatrics. 1989;84(1):28–35
  83. Global Tuberculosis Control: Surveillance, Planning Financing. Geneva, Switzerland: WHO; 2002;
  84. Engelbrecht AL, Marais BJ, Donald PR, Schaaf HS. A critical look at the diagnostic value of culture-confirmation in childhood tuberculosis. J Infect. 2006;53(6):364–369
  85. Perez Mato S, Van Dyke RB. Pulmonary infections in children with HIV infection. Semin Respir Infect. 2002;17(1):33–46
  86. Chintu C, Mudenda V, Lucas S, Nunn A, Lishimpi K, Maswahu D, et al. Lung diseases at necropsy in African children dying from respiratory illnesses: a descriptive necropsy study. Lancet. 2002;360(9338):985–990
  87. Palme IB, Gudetta B, Bruchfeld J, Muhe L, Giesecke J. Impact of human immunodeficiency virus 1 infection on clinical presentation, treatment outcome and survival in a cohort of Ethiopian children with tuberculosis. Pediatr Infect Dis J. 2002;21(11):1053–1061
  88. Rekha B, Swaminathan S. Childhood tuberculosis—global epidemiology and the impact of HIV. Paediatr Respir Rev. 2007;8(2):99–106
  89. Kenyon TA, Creek T, Laserson K, Makhoa M, Chimidza N, Mwasekaga M, et al. Risk factors for transmission of Mycobacterium tuberculosis from HIV-infected tuberculosis patients, Botswana. Int J Tuberc Lung Dis. 2002;6(10):843–850
  90. Mukadi YD, Wiktor SZ, Coulibaly IM, Coulibaly D, Mbengue A, Folquet AM, et al. Impact of HIV infection on the development, clinical presentation, and outcome of tuberculosis among children in Abidjan. Cote d'Ivoire AIDS. 1997;11(9):1151–1158
  91. Swaminathan S. Tuberculosis in HIV-infected children. Paediatr Respir Rev. 2004;5(3):225–230
  92. Ewer K, Deeks J, Alvarez L, Bryant G, Waller S, Andersen P, et al. Comparison of T-cell-based assay with tuberculin skin test for diagnosis of Mycobacterium tuberculosis infection in a school tuberculosis outbreak. Lancet. 2003;361(9364):1168–1173
  93. Graham SM, Coulter JB, Gilks CF. Pulmonary disease in HIV-infected African children. Int J Tuberc Lung Dis. 2001;5(1):12–23
  94. Perlman DC, el-Sadr WM, Nelson ET, Matts JP, Telzak EE, Salomon N, et al. Variation of chest radiographic patterns in pulmonary tuberculosis by degree of human immunodeficiency virus-related immunosuppression (The Terry Beirn Community Programs for Clinical Research on AIDS (CPCRA). The AIDS Clinical Trials Group (ACTG)). Clin Infect Dis. 1997;25(2):242–246
  95. Marais BJ, Graham SM, Cotton MF, Beyers N. Diagnostic and management challenges for childhood tuberculosis in the era of HIV. J Infect Dis. 2007;196(Suppl 1):S76–S85
  96. Du Toit G, Swingler G, Iloni K. Observer variation in detecting lymphadenopathy on chest radiography. Int J Tuberc Lung Dis. 2002;6(9):814–817
  97. Weismuller MM, Graham SM, Claessens NJ, Meijnen S, Salaniponi FM, Harries AD. Diagnosis of childhood tuberculosis in Malawi: an audit of hospital practice. Int J Tuberc Lung Dis. 2002;6(5):432–438
  98. Theart AC, Marais BJ, Gie RP, Hesseling AC, Beyers N. Criteria used for the diagnosis of childhood tuberculosis at primary health care level in a high-burden, urban setting. Int J Tuberc Lung Dis. 2005;9(11):1210–1214
  99. Kuhn JP, Silverman FN. Caffey's pediatric X-ray diagnosis. In: 9th edition. St. Louis (MO): Mosby; 1993;p. 666–671
  100. Leung AN, Muller NL, Pineda PR, FitzGerald JM. Primary tuberculosis in childhood: radiographic manifestations. Radiology. 1992;182(1):87–91
  101. Van Dyck P, Vanhoenacker FM, Van den Brande P, De Schepper AM. Imaging of pulmonary tuberculosis. Eur Radiol. 2003;13(8):1771–1785
  102. Andronikou S, Wieselthaler N. Modern imaging of tuberculosis in children: thoracic, central nervous system and abdominal tuberculosis. Pediatr Radiol. 2004;34(11):861–875
  103. Abadco DL, Steiner P. Gastric lavage is better than bronchoalveolar lavage for isolation of Mycobacterium tuberculosis in childhood pulmonary tuberculosis. Pediatr Infect Dis J. 1992;11(9):735–738
  104. Chan S, Abadco DL, Steiner P. Role of flexible fiberoptic bronchoscopy in the diagnosis of childhood endobronchial tuberculosis. Pediatr Infect Dis J. 1994;13(6):506–509
  105. Somu N, Swaminathan S, Paramasivan CN, Vijayasekaran D, Chandrabhooshanam A, Vijayan VK, et al. Value of bronchoalveolar lavage and gastric lavage in the diagnosis of pulmonary tuberculosis in children. Tuber Lung Dis. 1995;76(4):295–299
  106. Pomputius WF, Rost J, Dennehy PH, Carter EJ. Standardization of gastric aspirate technique improves yield in the diagnosis of tuberculosis in children. Pediatr Infect Dis J. 1997;16(2):222–226
  107. Lobato MN, Loeffler AM, Furst K, Cole B, Hopewell PC. Detection of Mycobacterium tuberculosis in gastric aspirates collected from children: hospitalization is not necessary. Pediatrics. 1998;102(4):E40
  108. Zar HJ, Hanslo D, Apolles P, Swingler G, Hussey G. Induced sputum versus gastric lavage for microbiological confirmation of pulmonary tuberculosis in infants and young children: a prospective study. Lancet. 2005;365(9454):130–134
  109. Vargas D, Garcia L, Gilman RH, Evans C, Ticona E, Navincopa M, et al. Diagnosis of sputum-scarce HIV-associated pulmonary tuberculosis in Lima, Peru. Lancet. 2005;365(9454):150–152
  110. Chow F, Espiritu N, Gilman RH, Gutierrez R, Lopez S, Escombe AR, et al. La cuerda dulce—a tolerability and acceptability study of a novel approach to specimen collection for diagnosis of paediatric pulmonary tuberculosis. BMC Infect Dis. 2006;6:67
  111. Steingart KR, Henry M, Laal S, Hopewell PC, Ramsay A, Menzies D, et al. A systematic review of commercial serological antibody detection tests for the diagnosis of extrapulmonary tuberculosis. Postgrad Med J. 2007;83(985):705–712
  112. Starke JR. Pediatric tuberculosis: time for a new approach. Tuberculosis (Edinb). 2003;83(1-3):208–212
  113. Lipsky BA, Gates J, Tenover FC, Plorde JJ. Factors affecting the clinical value of microscopy for acid-fast bacilli. Rev Infect Dis. 1984;6(2):214–222
  114. Yeager H, Lacy J, Smith LR, LeMaistre CA. Quantitative studies of mycobacterial populations in sputum and saliva. Am Rev Respir Dis. 1967;95(6):998–1004
  115. Hanna BA. Laboratory diagnosis. In:  Rom WN,  Garay SM editor. Tuberculosis. 2nd edition. Philadelphia (PA): Lippincott Williams and Wilkins; 2004;p. 164–176
  116. Stager CE, Libonati JP, Siddiqi SH, Davis JR, Hooper NM, Baker JF, et al. Role of solid media when used in conjunction with the BACTEC system for mycobacterial isolation and identification. J Clin Microbiol. 1991;29(1):154–157
  117. Morgan MA, Horstmeier CD, DeYoung DR, Roberts GD. Comparison of a radiometric method (BACTEC) and conventional culture media for recovery of mycobacteria from smear-negative specimens. J Clin Microbiol. 1983;18(2):384–388
  118. Roberts GD, Goodman NL, Heifets L, Larsh HW, Lindner TH, McClatchy JK, et al. Evaluation of the BACTEC radiometric method for recovery of mycobacteria and drug susceptibility testing of Mycobacterium tuberculosis from acid-fast smear-positive specimens. J Clin Microbiol. 1983;18(3):689–696
  119. Bird BR, Denniston MM, Huebner RE, Good RC. Changing practices in mycobacteriology: a follow-up survey of state and territorial public health laboratories. J Clin Microbiol. 1996;34(3):554–559
  120. Chan DS, Choy MY, Wang S, Sng LH. An evaluation of the recovery of mycobacteria from urine specimens using the automated Mycobacteria Growth Indicator Tube system (BACTEC MGIT 960). J Med Microbiol. 2008;57(Pt. 10):1220–1222
  121. Gray JW. Childhood tuberculosis and its early diagnosis. Clin Biochem. 2004;37(6):450–455
  122. Banaiee N, January V, Barthus C, Lambrick M, Roditi D, Behr MA, et al. Evaluation of a semi-automated reporter phage assay for susceptibility testing of Mycobacterium tuberculosis isolates in South Africa. Tuberculosis (Edinb). 2008;88(1):64–68
  123. Pai M, Kalantri S, Pascopella L, Riley LW, Reingold AL. Bacteriophage-based assays for the rapid detection of rifampicin resistance in Mycobacterium tuberculosis: a meta-analysis. J Infect. 2005;51(3):175–187
  124. Moore DA, Evans CA, Gilman RH, Caviedes L, Coronel J, Vivar A, et al. Microscopic-observation drug-susceptibility assay for the diagnosis of TB. N Engl J Med. 2006;355(15):1539–1550
  125. Caviedes L, Lee TS, Gilman RH, Sheen P, Spellman E, Lee EH, et al. Rapid, efficient detection and drug susceptibility testing of Mycobacterium tuberculosis in sputum by microscopic observation of broth cultures (The Tuberculosis Working Group in Peru). J Clin Microbiol. 2000;38(3):1203–1208
  126. Piersimoni C, Scarparo C. Relevance of commercial amplification methods for direct detection of Mycobacterium tuberculosis complex in clinical samples. J Clin Microbiol. 2003;41(12):5355–5365
  127. Gomez-Pastrana D. Tuberculosis in children—is PCR the diagnostic solution?. Clin Microbiol Infect. 2002;8(9):541–544
  128. Miotto P, Piana F, Penati V, Canducci F, Migliori GB, Cirillo DM. Use of genotype MTBDR assay for molecular detection of rifampin and isoniazid resistance in Mycobacterium tuberculosis clinical strains isolated in Italy. J Clin Microbiol. 2006;44(7):2485–2491
  129. Somoskovi A, Dormandy J, Mitsani D, Rivenburg J, Salfinger M. Use of smear-positive samples to assess the PCR-based genotype MTBDR assay for rapid, direct detection of the Mycobacterium tuberculosis complex as well as its resistance to isoniazid and rifampin. J Clin Microbiol. 2006;44(12):4459–4463
  130. Shinnick TM, Good RC. Diagnostic mycobacteriology laboratory practices. Clin Infect Dis. 1995;21(2):291–299
  131. Noordhoek GT, Kolk AH, Bjune G, Catty D, Dale JW, Fine PE, et al. Sensitivity and specificity of PCR for detection of Mycobacterium tuberculosis: a blind comparison study among seven laboratories. J Clin Microbiol. 1994;32(2):277–284
  132. Cheng VC, Yam WC, Hung IF, Woo PC, Lau SK, Tang BS, et al. Clinical evaluation of the polymerase chain reaction for the rapid diagnosis of tuberculosis. J Clin Pathol. 2004;57(3):281–285
  133. Sarmiento OL, Weigle KA, Alexander J, Weber DJ, Miller WC. Assessment by meta-analysis of PCR for diagnosis of smear-negative pulmonary tuberculosis. J Clin Microbiol. 2003;41(7):3233–3240
  134. Kafwabulula M, Ahmed K, Nagatake T, Gotoh J, Mitarai S, Oizumi K, et al. Evaluation of PCR-based methods for the diagnosis of tuberculosis by identification of mycobacterial DNA in urine samples. Int J Tuberc Lung Dis. 2002;6(8):732–737
  135. Rebollo MJ, San Juan Garrido R, Folgueira D, Palenque E, Diaz-Pedroche C, Lumbreras C, et al. Blood and urine samples as useful sources for the direct detection of tuberculosis by polymerase chain reaction. Diagn Microbiol Infect Dis. 2006;56(2):141–146
  136. Cannas A, Goletti D, Girardi E, Chiacchio T, Calvo L, Cuzzi G, et al. Mycobacterium tuberculosis DNA detection in soluble fraction of urine from pulmonary tuberculosis patients. Int J Tuberc Lung Dis. 2008;12(2):146–151
  137. Pai M. The accuracy and reliability of nucleic acid amplification tests in the diagnosis of tuberculosis. Natl Med J India. 2004;17(5):233–236
  138. Flores LL, Pai M, Colford JM, Riley LW. In-house nucleic acid amplification tests for the detection of Mycobacterium tuberculosis in sputum specimens: meta-analysis and meta-regression. BMC Microbiol. 2005;5:55
  139. Fauville-Dufaux M, Waelbroeck A, De Mol P, Vanfleteren B, Levy J, Debusschere P, et al. Contribution of the polymerase chain reaction to the diagnosis of tuberculous infections in children. Eur J Pediatr. 1996;155(2):106–111
  140. Delacourt C, Poveda JD, Chureau C, Beydon N, Mahut B, de Blic J, et al. Use of polymerase chain reaction for improved diagnosis of tuberculosis in children. J Pediatr. 1995;126(5 Pt. 1):703–709
  141. Smith KC, Starke JR, Eisenach K, Ong LT, Denby M. Detection of Mycobacterium tuberculosis in clinical specimens from children using a polymerase chain reaction. Pediatrics. 1996;97(2):155–160
  142. Johansen IS, Lundgren B, Tabak F, Petrini B, Hosoglu S, Saltoglu N, et al. Improved sensitivity of nucleic acid amplification for rapid diagnosis of tuberculous meningitis. J Clin Microbiol. 2004;42(7):3036–3040
  143. Arias M, Mello FC, Pavon A, Marsico AG, Alvarado-Galvez C, Rosales S, et al. Clinical evaluation of the microscopic-observation drug-susceptibility assay for detection of tuberculosis. Clin Infect Dis. 2007;44(5):674–680
  144. Shiferaw G, Woldeamanuel Y, Gebeyehu M, Girmachew F, Demessie D, Lemma E. Evaluation of microscopic observation drug susceptibility assay for detection of multidrug-resistant Mycobacterium tuberculosis. J Clin Microbiol. 2007;45(4):1093–1097
  145. Mello FC, Arias MS, Rosales S, Marsico AG, Pavon A, Alvarado-Galvez C, et al. Clinical evaluation of the microscopic observation drug susceptibility assay for detection of Mycobacterium tuberculosis resistance to isoniazid or rifampin. J Clin Microbiol. 2007;45(10):3387–3389
  146. Tovar M, Siedner MJ, Gilman RH, Santillan C, Caviedes L, Valencia T, et al. Improved diagnosis of pleural tuberculosis using the microscopic-observation drug-susceptibility technique. Clin Infect Dis. 2008;(in press)
  147. Martin A, Portaels F, Palomino JC. Colorimetric redox-indicator methods for the rapid detection of multidrug resistance in Mycobacterium tuberculosis: a systematic review and meta-analysis. J Antimicrob Chemother. 2007;59(2):175–183
  148. McNerney R, Traore H. Mycobacteriophage and their application to disease control. J Appl Microbiol. 2005;99(2):223–233
  149. Jacobs WR, Barletta RG, Udani R, Chan J, Kalkut G, Sosne G, et al. Rapid assessment of drug susceptibilities of Mycobacterium tuberculosis by means of luciferase reporter phages. Science. 1993;260(5109):819–822
  150. Alcaide F, Gali N, Dominguez J, Berlanga P, Blanco S, Orus P, et al. Usefulness of a new mycobacteriophage-based technique for rapid diagnosis of pulmonary tuberculosis. J Clin Microbiol. 2003;41(7):2867–2871
  151. Albert H, Trollip AP, Mole RJ, Hatch SJ, Blumberg L. Rapid indication of multidrug-resistant tuberculosis from liquid cultures using FASTPlaqueTB-RIF, a manual phage-based test. Int J Tuberc Lung Dis. 2002;6(6):523–528
  152. Muzaffar R, Batool S, Aziz F, Naqvi A, Rizvi A. Evaluation of the FASTPlaqueTB assay for direct detection of Mycobacterium tuberculosis in sputum specimens. Int J Tuberc Lung Dis. 2002;6(7):635–640
  153. Albert H, Heydenrych A, Brookes R, Mole RJ, Harley B, Subotsky E, et al. Performance of a rapid phage-based test (FASTPlaqueTB, to diagnose pulmonary tuberculosis from sputum specimens in South Africa). Int J Tuberc Lung Dis. 2002;6(6):529–537
  154. Albay A, Kisa O, Baylan O, Doganci L. The evaluation of FASTPlaqueTB test for the rapid diagnosis of tuberculosis. Diagn Microbiol Infect Dis. 2003;46(3):211–215
  155. Albert H, Heydenrych A, Mole R, Trollip A, Blumberg L. Evaluation of FASTPlaqueTB-RIF, a rapid, manual test for the determination of rifampicin resistance from Mycobacterium tuberculosis cultures. Int J Tuberc Lung Dis. 2001;5(10):906–911
  156. Krishnamurthy A, Rodrigues C, Mehta AP. Rapid detection of rifampicin resistance in m.tuberculosis by phage assay. Indian J Med Microbiol. 2002;20(4):211–214
  157. Riska PF, Jacobs WR. The use of luciferase-reporter phage for antibiotic-susceptibility testing of mycobacteria. Methods Mol Biol. 1998;101:431–455
  158. Riska PF, Su Y, Bardarov S, Freundlich L, Sarkis G, Hatfull G, et al. Rapid film-based determination of antibiotic susceptibilities of Mycobacterium tuberculosis strains by using a luciferase reporter phage and the Bronx Box. J Clin Microbiol. 1999;37(4):1144–1149
  159. Jacobs WR, Barletta RG, Udani R, Chan J, Kallkut G, Sosne G, et al. Rapid assessment of drug susceptibilities of Mycobacterium tuberculosis by means of luciferase reporter phages. Science. 1993;260:819–822
  160. Sada E, Aguilar D, Torres M, Herrera T. Detection of lipoarabinomannan as a diagnostic test for tuberculosis. J Clin Microbiol. 1992;30(9):2415–2418
  161. Tessema TA, Hamasur B, Bjun G, Svenson S, Bjorvatn B. Diagnostic evaluation of urinary lipoarabinomannan at an Ethiopian tuberculosis centre. Scand J Infect Dis. 2001;33(4):279–284
  162. Tessema TA, Bjune G, Hamasur B, Svenson S, Syre H, Bjorvatn B. Circulating antibodies to lipoarabinomannan in relation to sputum microscopy, clinical features and urinary anti-lipoarabinomannan detection in pulmonary tuberculosis. Scand J Infect Dis. 2002;34(2):97–103
  163. Boehme C, Molokova E, Minja F, Geis S, Loscher T, Maboko L, et al. Detection of mycobacterial lipoarabinomannan with an antigen-capture ELISA in unprocessed urine of Tanzanian patients with suspected tuberculosis. Trans R Soc Trop Med Hyg. 2005;99(12):893–900
  164. Choi SH, Kim YS, Bae IG, Chung JW, Lee MS, Kang JM, et al. The possible role of cerebrospinal fluid adenosine deaminase activity in the diagnosis of tuberculous meningitis in adults. Clin Neurol Neurosurg. 2002;104(1):10–15
  165. Gautam N, Aryal M, Bhatta N, Bhattacharya SK, Baral N, Lamsal M. Comparative study of cerebrospinal fluid adenosine deaminase activity in patients with meningitis. Nepal Med Coll J. 2007;9(2):104–106
  166. Riquelme A, Calvo M, Salech F, Valderrama S, Pattillo A, Arellano M, et al. Value of adenosine deaminase (ADA) in ascitic fluid for the diagnosis of tuberculous peritonitis: a meta-analysis. J Clin Gastroenterol. 2006;40(8):705–710
  167. Kashyap RS, Ramteke SP, Deshpande PS, Purohit HJ, Taori GM, Daginawala HF. Comparison of an adenosine deaminase assay with ELISA for the diagnosis of tuberculous meningitis infection. Med Sci Monit. 2007;13(9):BR200-4
  168. Al Zahrani K, Al Jahdali H, Poirier L, Rene P, Gennaro ML, Menzies D. Accuracy and utility of commercially available amplification and serologic tests for the diagnosis of minimal pulmonary tuberculosis. Am J Respir Crit Care Med. 2000;162(4 Pt. 1):1323–1329
  169. Mahadevan S. Clinical utility of serodiagnosis of tuberculosis. Indian J Pediatr. 1997;64(1):97–103
  170. Pottumarthy S, Wells VC, Morris AJ. A comparison of seven tests for serological diagnosis of tuberculosis. J Clin Microbiol. 2000;38(6):2227–2231
  171. Davidow A, Kanaujia GV, Shi L, Kaviar J, Guo X, Sung N, et al. Antibody profiles characteristic of Mycobacterium tuberculosis infection state. Infect Immun. 2005;73(10):6846–6851
  172. Kulshrestha A, Gupta A, Verma N, Sharma SK, Tyagi AK, Chaudhary VK. Expression and purification of recombinant antigens of Mycobacterium tuberculosis for application in serodiagnosis. Protein Expr Purif. 2005;44(1):75–85
  173. Singh KK, Dong Y, Patibandla SA, McMurray DN, Arora VK, Laal S. Immunogenicity of the Mycobacterium tuberculosis PPE55 (Rv3347c) protein during incipient and clinical tuberculosis. Infect Immun. 2005;73(8):5004–5014
  174. Silva VM, Kanaujia G, Gennaro ML, Menzies D. Factors associated with humoral response to ESAT-6, 38 kDa and 14 kDa in patients with a spectrum of tuberculosis. Int J Tuberc Lung Dis. 2003;7(5):478–484
  175. Bothamley GH, Rudd RM. Clinical evaluation of a serological assay using a monoclonal antibody (TB72) to the 38 kDa antigen of Mycobacterium tuberculosis. Eur Respir J. 1994;7(2):240–246
  176. Steingart KR, Henry M, Laal S, Hopewell PC, Ramsay A, Menzies D, et al. A systematic review of commercial serological antibody detection tests for the diagnosis of extrapulmonary tuberculosis. Thorax. 2007;62(10):911–918
  177. Raja A, Ranganathan UD, Bethunaickan R, Dharmalingam V. Serologic response to a secreted and a cytosolic antigen of Mycobacterium tuberculosis in childhood tuberculosis. Pediatr Infect Dis J. 2001;20(12):1161–1164
  178. Swaminathan S, Umadevi P, Shantha S, Radhakrishnan A, Datta M. Sero diagnosis of tuberculosis in children using two ELISA kits. Indian J Pediatr. 1999;66(6):837–842
  179. Wu X, Zhang L, Zhang J, Zhang C, Zhu L, Shi Y. Recombinant early secreted antigen target 6 protein as a skin test antigen for the specific detection of Mycobacterium tuberculosis infection. Clin Exp Immunol. 2008;152(1):81–87
  180. Arend SM, Franken WP, Aggerbeck H, Prins C, van Dissel JT, Thierry-Carstensen B, et al. Double-blind randomized Phase I study comparing rdESAT-6 to tuberculin as skin test reagent in the diagnosis of tuberculosis infection. Tuberculosis (Edinb). 2008;88(3):249–261
  181. Nakamura RM, Velmonte MA, Kawajiri K, Ang CF, Frias RA, Mendoza MT, et al. MPB64 mycobacterial antigen: a new skin-test reagent through patch method for rapid diagnosis of active tuberculosis. Int J Tuberc Lung Dis. 1998;2(7):541–546
  182. Nakamura RM, Einck L, Velmonte MA, Kawajiri K, Ang CF, Delasllagas CE, et al. Detection of active tuberculosis by an MPB-64 transdermal patch: a field study. Scand J Infect Dis. 2001;33(6):405–407
  183. Madebo T, Lindtjorn B. Delay in treatment of pulmonary tuberculosis: an analysis of symptom duration among Ethiopian patients. MedGenMed. 1999;E6
  184. Liam CK, Tang BG. Delay in the diagnosis and treatment of pulmonary tuberculosis in patients attending a university teaching hospital. Int J Tuberc Lung Dis. 1997;1(4):326–332
  185. Harries AD, Nyirenda TE, Godfrey-Faussett P, Salaniponi FM. Defining and assessing the maximum number of visits patients should make to a health facility to obtain a diagnosis of pulmonary tuberculosis. Int J Tuberc Lung Dis. 2003;7(10):953–958
  186. Xavier SMG. Acute bacterial meningitis beyond the neonatal period. In:  Long S editors. Long: principles and practice of pediatric infectious diseases. 3rd edition. Philadelphia (PA): Churchill Livingstone; 2008;
  187. Wallace R, Cohen AS. Tuberculous arthritis: a report of two cases with review of biopsy and synovial fluid findings. Am J Med. 1976;61(2):277–282
  188. Allali F, Mahfoud-Filali S, Hajjaj-Hassouni N. Lymphocytic joint fluid in tuberculous arthritis (A review of 30 cases). Joint Bone Spine. 2005;72(4):319–321

PII: S1538-5442(08)00134-X

doi: 10.1016/j.cppeds.2008.12.003

Current Problems in Pediatric and Adolescent Health Care
Volume 39, Issue 3 , Pages 61-88 , March 2009